Impact of glyphosate application to transgenic Roundup Ready soybean on horizontal gene transfer of the EPSPS gene to Bradyrhizobium japonicum and on the root-associated bacterial community
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Lack of glyphosate resistance gene transfer from Roundup Ready soybean to Bradyrhizobium japonicum under field and laboratory conditions.
A field study was conducted at the Russell E. Larson Agricultural Research Center to determine the effect of transgenic glyphosate-resistant soybean in combination with herbicide (Roundup) application on its endosymbiont Bradyrhizobium japonicum. DNA of bacteroids from isolated nodules was analysed for the presence of the transgenic 5-enolpyruvylshikimate-3-phosphate synthase (CP4-EPSPS) DNA se...
متن کاملThe Probability of a Horizontal Gene Transfer from Roundup Ready® Soybean to Root Symbiotic Bacteria: A Risk Assessment Study on the GSF Lysimeter Station
The gene transfer from glyphosate tolerant soybean to Bradyrhizobium japonicum was evaluated in a free-air lysimeter experiment under natural conditions and increasing selection pressure, to monitor for the probability of horizontal gene transfer (HGT). A large volume lysimeter study that offers conditions comparable to normal farming was conducted in 2004 and 2005 with Roundup Ready® (RR) soyb...
متن کاملImpact of glyphosate on the Bradyrhizobium japonicum symbiosis with glyphosate-resistant transgenic soybean: a minireview.
Glyphosate-resistant (GR) soybean [Glycine max (L.) Merr.] expressing an insensitive 5-enolpyruvylshikimic acid-3-phosphate synthase (EPSPS) gene has revolutionized weed control in soybean production. The soybean nitrogen fixing symbiont, Bradyrhizobium japonicum, possesses a glyphosate-sensitive enzyme and upon exposure to glyphosate accumulates shikimic acid and hydroxybenzoic acids such as p...
متن کاملGlyphosate Tolerance in Transgenic Canola by a Modified Glyphosate Oxidoreductase (gox) Gene
The engineering of transgenic canola (Brassica napus L. ) to make tolerance to the broad-spectrum herbicide, glyphosate, is one of the most effective approaches for weed management. Glyphosate inhibits the enzyme EPSPS (5-enolpyruvylshikimate-3-phosphate synthase) enzyme which functions in the shikimate pathway and has a key role in biosynthesis of aromatic amino acids required for survival of ...
متن کاملNitrogenase activity, nitrogen content, and yield responses to glyphosate in glyphosate-resistant soybean
Transgenic glyphosate-resistant (GR) soybean [Glycine max (L.) Merr.] expressing a glyphosate-insensitive 5-enolpyruvylshikimate-3phosphate synthase (EPSPS) enzyme has provided new opportunities for weed control in soybean production. However, glyphosate is toxic to the soybean nitrogen-fixing symbiont, Bradyrhizobium japonicum, as its EPSPS enzyme is sensitive to glyphosate. The effects of gly...
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